Fig. 3.

SAK3 treatment protects against apoptosis caused by TAF1 gene editing in the cerebral cortex. Apoptosis was assessed in somatosensory motor cortex pyramidal neurons in layer 5 samples using TUNEL assay. (A) Shown are photomicrographs from gRNA-TAF1 edited animals and gRNA-TAF1 edited animals treated with SAK3. The data from the control groups can be found in the Supplementary data. Note SAK3 reduced the number of TUNEL positive neuronal cells in gRNA-TAF1 edited animals. (B) Summary of the number of TUNEL positive cells in each of the experimental conditions. Data are shown as mean ± S.E.M., n = 12 fields per animal, 4 animals per experimental condition. *p < 0.05 versus; naïve and gRNA-TAF1 = SAK3 group (ANOVA followed by Tukey’s test). Scale bars: 200 μm. The experiments were conducted in an investigator-blinded manner.