Fig. 4.
The effects of SAK3 treatment on regulators of apoptosis in the cerebral cortex. (A) The effects of SAK3 administration on cleaved caspase 3 levels. Representative Western analysis is shown from cerebral cortex samples from animals from each of the experimental conditions. Note the increased cleaved caspase 3 in gRNA-TAF1 edited animals and diminished cleaved caspase 3 levels in gRNA-TAF1 SAK3 treated animals. (C) Blots show the increased BAX levels in gRNA-TAF1 edited animals, whereas SAK3 reduced BAX levels to control levels. (E) Blots show decreased Bcl-2 levels in gRNA-TAF1 edited animals, whereas SAK3 increase Bcl-2 expression to control levels. (B, D, F) Quantification of Western analysis from independent experiments. Data shown are mean ± SEM, n = 6 animals per each experimental condition. *p < 0.05 versus gRNA-control water and RNA-control SAK3 group; # p < 0.05 versus gRNA-TAF1 water group (ANOVA followed by Tukey’s test).