Fig. 6.

The effects of SAK3 treatment on AKT/GSKβ signaling in the cerebral cortex of TAF1 edited animals. (A) Expression of p-AKT was decreased in TAF1-edited animals (Av) as compared to all other experimental groups (Ai-iv). SAK3 administration to the TAF1 edited animals shows increased expression of p-AKT as compared to TAF1 edited group (Avi & v). (C) Representative Western analysis of p-GSK3β is shown from cerebral cortex samples from animals from each of the experimental conditions. Note decreased p-GSK3β levels in gRNA-TAF1 edited animals, whereas SAK3 increases p-GSK3β expression to control levels. (B) Summary of p-AKT expression, Data are shown as mean ± S.E.M., n = 12 fields per animal, 4 animals per experimental condition. Scale bars: 200 μm. (D) Quantification of Western analysis from three independent experiments. Data shown are mean ± SEM, n = 6 animals per each experimental condition. *p < 0.05 versus; naïve and gRNA-TAF1 = SAK3 group (ANOVA followed by Tukey’s test). The experiments were conducted in an investigator-blinded manner.