Table 1.
List of primary and secondary antibodies used in the study.
| Abbreviation | Primary antibodies |
|||||
|---|---|---|---|---|---|---|
| Anti- | Host | Company | Catalog number | Application | Dilution | |
| Bcl-2-associated X protein | Bax | Rabbit | Cell Signaling | 14796S | WB | 1:1000 |
| B-cell lymphoma 2 | Bcl2 | Rabbit | Abcam | Ab196495 | WB | 1:1000 |
| Brain-derived neurotrophic factor | BDNF | Mouse | Abcam | ab203573 | IHC | 1:500 |
| WB | 1:750 | |||||
| Cleaved Caspase 3 | C-Cas-3 | Rabbit | Cell Signaling | 9661S | WB | 1:1000 |
| Voltage-dependent T-type calcium channel subunit α1G | Cav3.1 | Rabbit | Almone labs | ACC-021 | IHC | 1:500 |
| Forkhead box protein P2 | FOXP2 | Rabbit | Abcam | Ab16046 | WB | 1:1000 |
| Glial fibrillary acidic protein | GFAP | Rabbit | Dako | Z0334 | IHC | 1:500 |
| phospho-Protein kinase B | p-AKT | Rabbit | Cell Signaling | 9271S | IHC | 1:500 |
| Phospho-Collapsin response mediator protein-2 | p-CRMP2 | Sheep | Kina source | PB-043 | WB | 1:1000 |
| Collapsin response mediator protein-2 | CRMP2 | Rabbit | Sigma | C2993 | WB | 1:1000 |
| Phospho-Glycogen synthase kinase 3 beta | p-GSK3β | Rabbit | Cell Signaling | 5558S | WB | 1:1000 |
| Glycogen synthase kinase 3 beta | GSK3β | Mouse | Cell Signaling | 9832S | WB | 1:1000 |
| Class III β-Tubulin | βIII-Tubulin | Mouse | Promega | G7121 | WB | 1:1000 |
| Anti- | Secondary antibodies |
|||||
|---|---|---|---|---|---|---|
| Label | Host | Company | Catalog number | Application | Dilution | |
| Rabbit IgG | HRP-linked | Goat | Cell Signaling | 7074S | WB | 1:3000 |
| Mouse IgG | HRP-linked | Horse | Cell Signaling | 7076S | WB | 1:3000 |
| Sheep IgG | IgG (H + L) | Donkey | Jackson ImmunoResearch laboratories. Inc | AB_2340704 | WB | 1:20000 |
| Rabbit IgG | Alexa 488 | Goat | Life technologies | A11034 | IHC | 1:500 |
| Mouse IgG | Alexa 488 | Rabbit | Life technologies | A21204 | IHC | 1.500 |
WB = Western blot; IHC = Immunohistochemistry;
a
Dilutions were from original stocks supplied by the vendors.