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Measurement of vascular density: (A) En face, optical coherence tomography angiography image of 3 × 3-mm2 disc area showing vessels from two combined layers; (B) Vessels were traced using Frangi vesselness plug-in; (C) Large vessel areas were selected using Tubness plug-in. Regions of interest were selected in the optic disc (D) and peripapillary area (E). Vascular density was calculated by dividing vessel area by region of interest.
