Figure 1.

Effects of HDM extract on IL-1α and IL-6 production by HaCaT and primary keratinocytes, and suppression by Aptamin C. HaCaT (A,C) and primary human keratinocytes (B,D) were treated with 25 μg/mL of HDM extract, with or without vitamin C (40 μM), Aptamin (0.09 μg/mL), or Aptamin C (vitamin C 40 μM/Aptamin 0.09 μg/mL) for 24 or 48 h. The culture supernatants were collected, and the concentrations of IL-1α (A,B) and IL-6 (C,D) were evaluated by ELISA as described in Materials and Methods. Data are presented as the mean ± SD. *** p < 0.001.