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. 2021 Jun 11;10(6):945. doi: 10.3390/antiox10060945

Figure 2.

Figure 2

Effects of HDM extract on T cell production of IL-17 and IL-22, and suppression by Aptamin C. T cells isolated from peripheral blood mononuclear cells (PBMCs) and purified with a MACS negative selection system, as described in Materials and Methods, and then treated with 25 μg/mL of HDM extract, with or without vitamin C (40 μM), Aptamin (0.09 μg/mL), or Aptamin C (Vitamin C 40 μM/Aptamin 0.09 μg/mL) for 48 or 72 h. The culture supernatants were collected, and the concentrations of IL-17 (A) and IL-22 (B) were measured by ELISA. Data are presented as the mean ± SD. *** p < 0.001.