Effects of hot-water extract of A. arabica bark on (A) membrane potential and (B) intracellular calcium in BRIN BD11 cells, (C–G) glucose uptake in differentiated 3T3L1 adipocyte cells, (H) acarbose, (I) starch digestion, (J) guar gum and (K) glucose diffusion in vitro. Changes in fluorescence intensity in differentiated 3T3L1 adipocytes incubated with extract in the (E) absence or (F) presence of 100 nM insulin. Images were taken at 10× magnification. Values are the mean ± SEM for n = 6 for membrane potential and intracellular calcium and n = 4 (~20 values of fluorescence intensity per group for glucose uptake) for glucose uptake, starch digestion and glucose diffusion. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to control.