Figure 7.

Representative (A) HPLC profile and (B) insulin-releasing effects in BRIN BD11 cells of peak samples (1–5) of hot-water extract of A. arabica bark. The crude extract was chromatographed at a flow rate of 1.0 mL/min on a (10 × 250 mm) semipreparative 5 μm C-18 column (Phenomenex, UK). The concentration of the eluting solvent was raised using linear gradients of acetonitrile (0–20% from 0 to 10 min, 20–70% from 10 to 40 min and 70–20% from 40 to 60 min). Compounds were detected by measurement of absorbance at 254 nm, and major peaks labelled P1-P5 were assessed for insulin-releasing activity. Values are the mean ± SEM for n = 8. *** p < 0.001 compared to control.