Table 1.
Molecular mass and predicted identity of peak samples of A. arabica bark obtained from the preparative RP-HPLC via LC-MS analysis.
| Peak Samples | Retention Time (min) |
Theoretical Molecular Wt. (Da) |
Found Molecular Weight (Da) |
Predicted Compounds |
|---|---|---|---|---|
| P1 | 9.7 | 302.2 | 301.2 | Quercetin |
| P2 | 19 | 290.3 | 289.0 | Catechin |
| P3 | 23 | 286.2 | 285.2 | Kaempferol |
| P4 | 29 | - | 677.2 | Unknown |
| P5 | 32.5 | - | 496.9 | Unknown |
Peaks were separated on a Spectra System LC using a Kinetex 5 µm F5 LC column (150 × 4.6 mm2) (Phenomenex). The mass-to-charge ratio (m/z) versus peak intensity was determined.