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. 2021 Jun 11;10(6):944. doi: 10.3390/antiox10060944

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Luciferase gene reporter assay based on the activation of the Nrf2/ARE signaling pathway. (1) Constitutive proteasome degradation of Nrf2 protein is inhibited by inducers acting on KEAP1 stress sensors (cysteines), leading to free cytosolic Nrf2; (2) Nrf2 is transferred to the nucleus where it activates antioxidant response element (ARE)-dependent gene transcription; (3) expression of luciferase as gene reporter; (4) luciferase-dependent formation of luminescent oxyluciferin from luciferin after cell lysis.