Table 2.
The combination index values (CI < 0.8: synergism; 0.8 < CI < 1.2: additive effect; and CI > 1.2: antagonism), and combined estrogenic effects of ZEN with ZELs, ZAN, ZALs, and GEN.
| 6.28 × 10−2 µM ZEN + |
CI Value | Combined Estrogenic Effect | |
|---|---|---|---|
| 6.24 × 10−3 µM α-ZEL | 0.37 | Synergism | ++ |
| 1.25 × 10−2 µM α-ZEL | 0.41 | Synergism | ++ |
| 1.22 µM β-ZEL | 0.77 | Synergism | + |
| 2.43 µM β-ZEL | 0.95 | Additive effect | ± |
| 2.50 × 10−2 µM ZAN | 0.58 | Synergism | ++ |
| 4.99 × 10−2 µM ZAN | 0.62 | Synergism | + |
| 1.24 × 10−2 µM α-ZAL | 0.97 | Additive effect | ± |
| 2.48 × 10−2 µM α-ZAL | 1.16 | Additive effect | ± |
| 1.24 × 10−1 µM β-ZAL | 1.19 | Additive effect | ± |
| 2.48 × 10−1 µM β-ZAL | 1.56 | Antagonism | -- |
| 7.4 × 10−1 µM GEN | 0.58 | Synergism | ++ |
| 1.48 µM GEN | 0.77 | Synergism | + |
ZEN, zearalenone; α-ZEL, α-zearalenol; β-ZEL, β-zearalenol; ZAN, zearalanone; α-ZAL, α-zearalanol; β-ZAL, β-zearalanol; GEN, genistein; CI < 0.60: ++; 0.60 < CI < 0.80: +; 0.80 < CI < 1.20: ±; 1.20 < CI < 1.40: -; and CI > 1.40: --.