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Calcium flux by VRC01-expressing B cells over time in response to decreasing concentrations of soluble Env, SiNP100-Env, and SiNP200-Env. (a–d) Activation of B cells at different protein concentrations of soluble or particle-bound Env. (e) SiNP100 particles and SiNP200 particles were used as controls and did not show any activation. Data shown is representative out of two separate assays.
