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. 2021 Jun 11;10(6):1465. doi: 10.3390/cells10061465

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Tripolar division of a hybrid cell. M13SV1 human breast epithelial cells [108] were either stably transduced with pH2B-GFP (kind gift from Geoff Wahl; Addgene plasmid #11680; http://n2t.net/addgene:11680; RRID: Addgene_11680; accessed on 15 April 2021) or pH2B_mCherry_IRES_puro2 (kind gift from Daniel Gerlich; Addgene plasmid #21045; http://n2t.net/addgene:21045; accessed on 15 April 2021, RRID:Addgene_21045). Hybrid cells were cultured on chamber slides (ThermoFisher Scientific GmbH, Schwerte, Germany) and time-lapse series were recorded using a Leica TCS SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany).