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. 2021 Jun 11;14(6):558. doi: 10.3390/ph14060558

Figure 3.

Figure 3

Immunohistochemical localization of HMGB1 expression (red) within the median preoptic nucleus (MnPO) and area postrema (AP) 24 h after injection of PBS or LPS. Rats treated with PBS showed intranuclear HMGB1 signals in both structures (A, a + B, b; open white arrowheads). After injection of LPS (10 mg/kg (C, c + D, d) or 1 mg/kg (E, e + F, f)) HMGB1 signals were detected perinuclearly within the cytoplasm (filled white arrowheads). Specific staining disappeared in technical controls (Supplementary Figure S1). The inserted boxes represent magnified images of marked areas (white dashed rectangles). Four mice per group of treatment (PBS, LPS 10 mg/kg, LPS 1 mg/kg) with at least two slides per animal were stained and representative pictures are displayed. Scale bars represent 100 µm (A) or 25 µm (a) and are applicable for all images.