Figure 3.

The prominent role of TIPRL in huh7, HCC, cell viability and stemness. Chang (A,C) and huh7 (A–F) cells were cultured, and then cells were seeded in a 96-well plate (A) or Anoikis plates (B,D–F) followed by transfected with the indicated siRNAs (100 nM). For cell proliferation (A) and viability (B) assays, 48 h after siRNA transfection, an MTT assay was performed. For quantification analysis of expression in stemness-related genes, we performed RT-qPCR using primers (C,D; Table S12). (E,F) We counted the numbers of spheroids after 72 h siRNAs transfection. TSFE, tumorsphere formation efficiency: [(the number of tumorspheres formed/the initial number of cells seeded) × 100]. All experiments were independently repeated three times. ** p < 0.01, *** p < 0.001 by unpaired t-test. ns, not significant.