Figure 5.

Effect of single-dose and repeated-dose minocycline and duloxetine, each used alone, on pain threshold in oxaliplatin-treated mice. Effect of minocycline (50, 100 mg/kg, i.p.) on cold hyperalgesia was measured in the cold plate test in mice on the day of oxaliplatin (oxa) administration (A) and 7 days later (B). Effect of duloxetine (10, 30 mg/kg, i.p.) on cold hyperalgesia was measured in the cold plate test in mice on the day of oxaliplatin administration (C) and 7 days later (D). The results are shown as the mean latency to pain reaction [s] ± SEM for n = 9–10. Statistical analysis: repeated measures ANOVA followed by Tukey’s post-hoc comparison. Significance: ### p < 0.001 vs. before oxaliplatin; * p < 0.05 vs. pre-drug latency to pain reaction in the individual group. In the figure titles ‘early phase’ (A,C) and ‘late phase’ (B,D) mean two time points at which the measurements were taken (day of oxaliplatin administration and day 7 after a single-dose oxaliplatin administration, respectively). Numerical values of the latencies to pain reaction ± SEM presented in this figure are shown in the Supplementary Materials (Table S3).