Table 1. Comparison of Vr3a and other typical M-superfamily conotoxins.
| Name | GenBank number | Signal region | Pro-region | Mature region |
|---|---|---|---|---|
| M-superfamily conotoxins from Conus varius | ||||
| Vr3e | AEX60121.1 | MLKMGVVLFIFLVLFPLATLQLDA | DQPVERYAENKQLLNTDERREIILSALR | -RQ CC DSNS C EYPK C L--- CC NG |
| Vr3d | AEX60202.1 | MLKMGVVLFTFLVLFPLATLQLDA | DQPVERNAENKQDINPDERRGFITLALR | HRGCCPIGPC LQSV C SP-- CC P- |
| Vr3c | AEX60204.1 | MLKMGVVLFTFLVLFPLARLQLDA | DQPVERNAENKQDINPDERKRFLTLALR | QGR CCPYGPC RLSM C DPP CC A- |
| Vr3b | AEX60198.1 | MLKMGVVLFTFLVLFPLATLQLDA | DQPVERYAENKQDINPDERKAFITLALG | QEGCCPSGPC HFAA C NPP- CC T- |
| Vr3a | AEX60199.1 | MLKMGVVLFTVLVLFPLATLHLDA | EQPVERYAENKQDINPDQRSGFLTFALR | QGCCPPGV C QMAA C NPPP CCP- |
| M-superfamily conotoxins from other Conus species | ||||
| tx3a | Q9BH73 | MLKMGVVLFIFLVLFPLATLQLDA | DQPVERYAENKQLLSPDERREIILHALG | TRCCSWDVCDHPSCTCCG |
| mr3e | Q5EHP3 | MLKMQVVLFIVLVLFPLATLQLDA | DQPVERYAENKRLLNPDERRGIILHALG | GRVCCPFGGCHELCYCCDG |
| Tx3b | P0C1N8 | MSKLGALLTICLLLFSLTAVPLDGDQH | ADQPAQRLQDRIPTEDHPLFDPN | KRCCPPVACNMGCKPCCG |
| GIIIA | P01523 | MMSKLGVLLTICLLLFPLTALPMDGDEP | ANRPVERMQDNISSEQYPLFEKR | RDCCTPPKKCKDRQCKPQRCCAGR |
| SIIIA | Q86DU6 | MMSKLGVLLTVCPLLFPLTALPPDGDGP | ADRPAEEMQDDISSDEHPLFDKR | QNCCNGGCSSKWCRDHARCCGR |
For the M-superfamily conotoxins from Conus varius, the nonconservative amino acids are marked in red and the amino acids in the third cysteine loop are marked in green. Cysteines in the mature region are in bold for all the conotoxins.