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. 2021 Jun 11;10(6):949. doi: 10.3390/antiox10060949

Figure 3.

Figure 3

Quantitative results of flow cytometry analysis of drug influx and efflux in MDA-MB-231 and MCF7 cells. (A,B) Elevated drug influx (original and remaining) and efflux (original quantity minus remaining) were observed in DEHP-exposed MDA-MB-231 cells compared with parental MDA-MB-231 cells. (C,D) Decreased drug influx and efflux were observed in MCF-7 cells (parental vs. DEHP-exposed). (E,F) Quantitative analysis of mRNA levels showed no significant change in MRP1/ABCC1 and high MDR1/ABCB1 expression. (G) Representative western blot results show the overexpression of MRP1/ABCC-1 and MDR1/ABCB-1 in the long-term DEHP-exposed clones compared to the control and DoxR MDA-MB-231 cells. * p < 0.05, ** p < 0.001. ■ represent Dox uptake for 2 h, ■ represent Dox efflux for 1 h, □ represent intrinsic/basal fluorescence considered as background.