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. 2021 Jun 11;10(6):949. doi: 10.3390/antiox10060949

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative results of the microwestern array and western blotting. (A) Target proteins of different signaling pathways, such as apoptosis, PI3K/Akt, and NFκB, were evaluated in control/parental MDA-MB-231, DoxR, and DEHP-exposed clones #1, 2, 3, and 4. Downregulation of PTEN, GSK3β, and BAX was observed in DEHP-exposed clones. The expression levels of different genes are represented by different colors from the control. Green represents downregulation with a minimum score of -1.17, and red represents upregulation with a maximum score of +1.17. (B) Representative western blot results show activation of PI3K/Akt signaling in DEHP exposed MDA-MB-231 cells. Upregulation of PI3K p85, Phospho-PI3K p85 (Tyr458), Phospho-AKT (Ser473), Phospho-GSK3β (Ser9), and Phospho-S6 (Ser235/236) was observed along with slight downregulation of AKT in clone #2 and downregulation of GSK3β in DEHP exposed clone #1 and #2.