Figure 2.
Structure of T-DNA regions of different vector systems. (a) The T-DNA fragment of plasmid pWEN 100 harbors the hygromycin-resistant marker gene (hptII) and the reporter gene (egfp) controlled by CaMV35S promoters. (b) The T-DNA region of plasmid pWEN 200 harbors the hptII gene and the reporter gene gus controlled by CaMV35S promoters. (c) The T-DNA region of plasmid pWEN 650 harbors the hptII-2A-gus open reading frame (ORF) controlled by the CaMV35S promoter and 5′UTR of the light-harvesting chlorophyll-binding protein of photosystem II (LHCPII) of E. gracilis. A copy of the first intron of LHCPII is placed in the hptII gene keeping the amino acid sequence unchanged. LB: Left border of T-DNA, RB: right border of T-DNA, CaMV35S: cauliflower mosaic virus 35S promoter, hptII: hygromycin phosphotransferase II, NOS: nopaline synthase terminator, egfp: enhanced green fluorescent protein, gus: β-glucuronidase, F2A: foot and mouth disease virus-derived (FMDV) 2A.