Figure 4.

Cell wall association of SYFO1 is essential for its function

(A and B) The constitutively expressed ECD of SYFO1 labeled the cell periphery in non-plasmolyzed cells (A) and remained at the cell wall upon plasmolysis (B). Arrowheads and arrows mark the cell wall and the retracted plasma membrane, respectively.

(C–F) FRAP experiments on roots hairs revealed a low mobility of full-length SYFO1 (C and E), although deletion of the PRR resulted in an increased mobility of the protein (D and F).

(G) Quantification of the mobile fractions of SYFO1 (n = 17 regions of interest [ROIs] from 4 independent plants) and SYFO1^ΔPRR (n = 12 ROIs from 4 independent plants); asterisks indicate a significant statistical difference based on a Student’s t test.

(H) The SYFO1^ΔPRR variant failed to genetically complement both syfo1 mutant alleles in comparison to roots transformed with the empty vector (ev) scoring n = 10 independent root systems per genotype.

Asterisks indicate a significant statistical difference based on a Tukey-Kramer multiple-comparison test with ^∗∗∗p < 0.001 and ^∗∗p < 0.01. Data are shown as mean ± SE. Scale bars indicate 10 μm (A–D). See also Figure S4.