Figure 7.
Effect of the cell culture insert circumference on the transendothelial electrical resistivity (TEER). (a) TEER measured with chopstick electrodes on immortalized mouse cerebellar capillary endothelial cells (cerebEND) using different sizes of cell culture inserts. Means ± SEM, n = 15–76, one-way ANOVA with Bonferroni post-test; ### p < 0.001, between 24 mm and 6.5 mm cell culture inserts; *** p < 0.001, between 24 mm and 12 mm cell culture inserts; §§§ p < 0.001, between 12 mm and 6.5 mm cell culture inserts. (b) TEER measured on an intestinal barrier model on different sizes of cell culture inserts both with the chopstick and the chamber electrode setup. Means ± SEM, n = 6, two-way ANOVA with Bonferroni post-test; ΔΔΔ p < 0.001, between chopstick and chamber electrodes; *** p < 0.001, between 24 mm and 12 mm cell culture inserts; § p < 0.05, §§ p < 0.01, between 12 mm and 6.5 mm cell culture inserts.