Figure 2.

Analysis of extracted RNA: (A) concentrations of total RNA extracted from live-cell cultures were similar among HGF-1, SCC4, SCC9, SCC15, SCC25, and CAL27 cells ranging from 314 to 369 ng/uL, p = 0.2939; (B) average RNA purity (A260:A280 ratio) plotted against RNA concentration. Average absorbance ratio measured at A260 nm and A280 nm revealed ranges between 1.69 and 1.91, with no significant difference between cell lines or discernable associations with overall concentration, p = 0.784.