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. 2021 Jun 11;22(12):6295. doi: 10.3390/ijms22126295

Figure 3.

Figure 3

Validation efficiency for upregulated gene expression prioritized by IPA analysis. (A) The PPARD upstream regulator identified by URA analysis is upregulated in dexamethasone-treated mouse cerebrocortical cultures (Care4Rare Neuron Screen). Orange arrows signify an activating relationship with downstream genes and red symbols signify upregulated genes with the Neuron Screen Z-score and p-adj appearing directly below each symbol. Only genes for which p-adj < 0.05 are included. (B–E) Adult male C57BL6 mice were treated p.o. with vehicle or DEX (1 mg/kg) for 5 days, and qRT-PCR was employed to determine gene expression of 7 of the 10 targets of PPARD (n = 3). The four genes with the most robust upregulation are shown. (B) Bcl-2-like protein 1 (Bcldl1). (C) Integrin-linked kinase (Ilk). (D) Major facilitator superfamily domain containing 2A (Mfsd2a). (E) Pyruvate dehydrogenase kinase 4 (Pdk4). For all hits, statistical significance was measured by Student’s paired two-tailed t-test (** p < 0.01).