Table 1.

Summary of six upstream regulators identified in the Neuron Screen data.

Drug Name	Upstream Regulator	Expression Fold Change	Predicted Activation State	Activation Z-Score	p-Value of Overlap	Number of Genes
Levothyroxine	DIO3	4.385	Inhibited	−2.975	1.79 × 10−7	8
Hydroxyurea	FOXM1	−3.579	Inhibited	−3.245	1.63 × 10−10	8
Dexamethasone	PPARD	2.522	Activated	3.126	4.58 × 10−2	10
Dexamethasone	STAT4	NA	Activated	2.933	7.36 × 10−4	12
Vigabatrin	MKNK1	1.187	Inhibited	−3	1.40 × 10−4	5
Pregabalin	PGR	1.013	Activated	3.376	7.77 × 10−9	13

The expression fold change indicated is derived from the original study reporting the RNA-seq results from the neuronal screen. The level of mRNA (i.e., RNA-seq read numbers) for a given gene in the presence of a drug is compared against the average mRNA level of that gene for all conditions while the upstream regulator is the central molecule in the network. The activation z-score serves as a statistical measure as well as a directional tool (negative/downregulated, positive/upregulated) while the p-value of overlap serves as an additional measure of statistical certainty pertaining to the interrelated nature of the molecules forming each network. NA, data not available as gene expression was not included in the Neuron Screen data [25].