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. 2021 Jun 12;26(12):3602. doi: 10.3390/molecules26123602

Figure 2.

Figure 2

STING expression in IHH cells pretreated or not with sulforaphane (SFN, 10 µM) in presence of inflammatory stimulus cGAMP (5.9 µM). Data are shown as means and C.I. of two representative experiments and reported evaluating 2−ΔΔCt values using untreated cells as calibrator and HPRT1 and G6DP housekeeping genes as reference. *: p <0.05, one way ANOVA untreated CTRL IHH cells vs. 72 h SFN 10 µM treatment. ****: p < 0.0001, one way ANOVA IHH exposed to 5.9 µM cGAMP for 3 h and vs. IHH pre-treated with SFN 10 µM for 72 h and 5.9 µM cGAMP for 3 h. ***: p < 0.001, one way ANOVA IHH exposed to 5.9 µM cGAMP for 6 h vs. IHH pre-treated with SFN 10 µM for 72 h and 5.9 µM cGAMP for 6 h. ****: p < 0.0001, one way ANOVA untreated CTRL IHH cells vs. IHH treated with 5.9 µM cGAMP for 3 and 6 h.