Figure 4.

GSTM1 expression in IHH cell line treated with sulforaphane (SFN, 10 µM) for 24 h (a) and 72 h (b). Data are shown as means and C.I. of three representative experiments and reported evaluating 2^−ΔΔCt values, using untreated cells as calibrator (CTRL) and beta-actin housekeeping gene as reference. ****: p < 0.001, one way ANOVA IHH exposed to SFN vs. untreated CTRL IHH cells.