Table 8.
Universal Probe Library (UPL) probes (Roche) and primers (Eurofins Genomics) for the six interferon-stimulated genes (ISGs) of interferon signature and STING analysis by Real-Time PCR.
| Probes and Primers | |||
|---|---|---|---|
| GENE | Probe (10 μM) |
Primer Forward (20 μM) |
Primer Reverse (20 μM) |
| ISGs assessed for interferon signature analysis | |||
| IFI27 | P. n. 21 | GTGGCCAAAGTGGTCAGG | CCAATCACAACTGTAGCAATCC |
| IFI44L | P. n. 15 | TGACACTATGGGGCTAGATGG | TTGGTTTACGGGAATTAAACTGAT |
| IFIT1 | P. n. 82 | TCCACAAGACAGAGAATAGCCAGAT | GCTCCAGACTATCCTTGACCTG |
| ISG15 | P. n. 76 | GAGGCAGCGAACTCATCTTT | AGCATCTTCACCGTCAGGTC |
| RSAD2 | P. n. 76 | ACAAATGCGGCTTCTGTTTC | GAAATGGCTCTCCACCTGAA |
| SIGLEC1 | P. n. 76 | CTGCCCTGCAAGTCCTCTA | CAGCAGGTGGCTCACTGTC |
| Primers and probe for measuring STING expression | |||
| STING | P. n. 51 | CGCCTCATTCCCTACCAG | TGCCCACAGTAACCTCCTCC |
| Housekeeping genes | |||
| HPRT1 | P. n. 73 | TGACCTTGATTTATTTTGCATACC | CGAGCAAGACGTTCAGTCCT |
| G6PD | P. n. 82 | GCAAACAGAGTGAGCCCTTC | GAGTTGCGGGCAAAGAAGT |