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ICT testing against the serum panel. The panel sera are from 3 humans (HuP1–3) and 3 horses (HsP1–3) with pythiosis, 5 horses with another infection caused by B. ranarum (HsB1–3), Aspergillus flavus (HsC4), and Actinomyces species (HsC5), 4 healthy individuals (HuC1, HsC1–3), and 1 P. insidiosum CFA-immunized rabbit (RbCFA). Black arrows indicate the control and test lines of the ICT assay. White arrows show the generated test line of each ICT-positive serum sample.
