Figure 3.

Effects of iron on the transcription of cosR and perR over the growth of C. jejuni. The P_cosR::lacZ and P_perR::lacZ fusion assays were conducted in the presence and absence of iron in MEMα and MH media: (a) P_cosR::lacZ assay in MEMα. (b) P_perR::lacZ in MEMα. (c) P_cosR::lacZ assay in MH. (d) P_perR::lacZ in MH. Deferoxamine mesylate (DFMS) was used as an iron chelator in MH media. The growth of C. jejuni is indicated with the optical density at 600 nm (OD₆₀₀) in each panel. The experiment was repeated three times, and similar patterns of results were obtained in the experiments. The statistical analysis was performed using Student’s t-test by comparing the samples with and without iron at the same sampling point. *: p < 0.05, **: p < 0.01, ***: p < 0.001.