Figure 3.

Insulin genes and secretion. RIN-m(^{-/- Adcy7}) mRNA level established by quantitative RT-PCR. The CT values were normalized to β-actin to yield ΔCT and 2^-ΔΔCT values presented in dots and bar chart, respectively. Each dot represents an individual sample, whereas the horizontal lines represent the mean and standard error of the mean. ΔCT values were then calculated and presented as 2^-ΔΔCT values (fold change) in a bar chart. (A) presents the mRNA level of Ins1 when incubated in low glucose level 2mM and high glucose level 10mM, while (B) shows the expression level of Ins2 under the same condition. (C) illustrates insulin secretion when incubated for one hour with 2mM glucose and 10mM glucose compared to the control. Unpaired t-test with mean of ELISA or delta CT and SE ± values were used for statistic test.