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. 2021 Jun 11;12:657873. doi: 10.3389/fendo.2021.657873

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Copyright © 2021 Alhaidan, Christesen, Lundberg, Balwi and Brusgaard

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exome data analysis strategy. RIN-m^(-/- ^Adcy7 ⁾ mRNA level under 2mM glucose and 10mM glucose concentration established by quantitative RT-PCR. The CT values were normalized to β-actin to yield ΔCT and 2^-ΔΔCT values presented in dots and bar chart, respectively. Each dot represents an individual sample, whereas the horizontal lines represent the mean and standard error of the mean. ΔCT values were then calculated and presented as 2^-ΔΔCT values (fold change) in a bar chart. (A) PRKACs alpha (Prkaca) and beta (Prkacb) expressions, (B) Rapgef4, (C) Slc2a2, (D) Gck, (E) Pdx1, (F) Foxa2. Unpaired t-test with delta CT and SE ± values were used for statistic test.