KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-DNA Polymerase theta antibody	Abcam	Cat#ab80906
Mouse anti-rabbit IgG-HRP	Santa Cruz	Cat#sc-2357
Bacterial and Virus Strains
Rosetta 2(DE3)pLysS Competent Cells	Millipore Sigma	Cat#70956-3
Stellar Competent Cells	Takara Bio	Cat#636766
Chemicals, Peptides, and Recombinant Proteins
Roche Deoxynucleoside Triphosphate Set	Millipore Sigma	Cat#11969064001
Sequel NE Reagent A	American Bio	Cat#AB13021-01000
Sequel NE Reagent B	American Bio	Cat#AB13022-01000
T4 PNK	New England Biolabs	Cat#M0201S
γ-32P-ATP	Perkin Elmer	Cat#BLU502Z500UC
Ni-NTA resin	Qiagen	Cat#30210
GE HiTrap Heparin column	VWR	Cat#95055-850
GE Superose 6 Increase 10/300 GL	VWR	Cat#10192-228
GE Superdex 200 10/300 GL	VWR	Discontinued. New Model: Cat#89497-272
TCEP solution	Millipore Sigma	Cat#646547
GenElute HP Select Plasmid Gigaprep Kit	Millipore Sigma	Cat#NA0800-1KT
Polyethylenimine	Polysciences	Cat#23966
Gibco CD Hybridoma media	ThermoFisher Biosciences	Cat#11279023
Roche cOmplete Protease Inhibitor	Millipore Sigma	Cat#11873580001
Clarity ECL	BioRad	Cat#1705060
T4 Ligase	New England Biolabs	Cat#M0202S
Deposited Data
Crystal structure of pol QM1	Protein Data Bank	PDBID#4X0P
Experimental Models: Cell Lines
Expi293F Cells	ThernoFisher Scientific	Cat#A14527
Oligonucleotides
ss14AG	Synthetic oligonucleotide	5’-GCGGCTGTCATAAG
ss14GC	Synthetic oligonucleotide	5’-GCGGCTGTCATACG
Recombinant DNA
Sumo3 PolQM1	Addgene	Cat#78462
Software and Algorithms
Bowtie2	Nature Methods. 2012, 9:357–359.	N/A
Pear	Bioinformatics. 2014 Mar 1; 30(5): 614–620.	N/A
Bam-Readcount	https://github.com/genome/bam-readcount	N/A