Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 22;43(1):968–979. doi: 10.1080/0886022X.2021.1936040

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Effects of ATF4 knockdown and HO-1 overexpression on podocyte autophagy and apoptosis when subjected to serum from DN mice. (A) HO-1 overexpression was verified in MCP-5 podocytes. (B) Podocytes overexpressing HO-1 were subjected to serum from DN mice, showed significantly reduced cleaved caspase 3 expression, and co-treatment of HO-1 overexpression with ATF4 siRNA increased cleaved caspase-3 expression. (C) Densitometric analysis of protein expression from Figure 5(B). (D) Immunofluorescence analysis showed increase in autophagy LC3 protein expression by HO-1 overexpression, which was significantly reduced by ATF4 siRNA co-treatment; the scale indicates 10 μm. (E) Quantification of apoptosis rates by flow cytometry supported our western analysis of cleaved caspase-3. (F) Apoptosis was evaluated by flow cytometry. NC siRNA: control siRNA transfected for 48 h; ATF4 siRNA: siRNA-ATF4 transfected for 48 h. Vector: Empty plasmid transfected for 48 h. HO-1 OE: HO-1 overexpression plasmid transfected for 48 h. (**p < 0.01, *p < 0.05).