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. 2021 Jun 22;43(1):968–979. doi: 10.1080/0886022X.2021.1936040

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effects of HO-1 agonist hemin treatment on clinical indications of DN-induced autophagy and apoptosis in podocytes. (A) Evaluation of urinary total protein and urinary albumin showed increased content in DN mice compared to healthy controls, while hemin treatment significantly reduced proteinuria and albuminuria in DN mice. (B) Serum creatinine and urea levels were also significantly reduced by hemin treated DN mice. (C) PAS staining showed glomerular damage in DN mice and it was rescued by hemin treatment. (D) Protein expression analysis revealed that expression of critical protein in renal filtration barrier nephrin was downregulated in DN mice, and hemin treatment significantly increased the neprhin expression and densitometric analysis of protein expression. (E) TUNEL staining showed that hemin treatment significantly reduced apoptotic cells in DN mice. (F) Quantification of apoptotic cells. (G) Protein expression by Western blot. (H) Densitometric analysis of protein expression. (I)Expression and distribution of nephrin and LC3 protein were evaluated by immunofluorescence; the scale indicates 50 μm. (**p < 0.01, *p < 0.05).