. 2021 Jun 25;87(14):e02839-20. doi: 10.1128/AEM.02839-20

TABLE 3.

Stoichiometry and Gibbs free energy change of the reactions involved in the coculture of Ac. wieringae strain JM and An. neopropionicum metabolism on CO and acetate

Organism	Reaction	Value of Gibbs free energy change (kJ)^a	Equation
Ac. wieringae strain JM	4H₂ + 2CO₂ → acetate⁻ + H⁺ + 2H₂O	−95	1
	4CO + 2H₂O → acetate⁻ + H⁺ + 2CO₂	−174	2
	6CO + 3H₂O → ethanol + 4CO₂	−224	3
	Acetate⁻ + H⁺ + 2H₂ → ethanol + H₂O	−9.6	4

An. neopropionicum	3 ethanol + 2HCO₃⁻ → 2 propionate⁻ + acetate⁻ + H⁺ + 3H₂O	−124	5
	3H₂ (6H) + acetate⁻ + H⁺ + HCO₃⁻ → propionate⁻ + 3H₂O	−76	6
	Ethanol + acetate⁻ → butyrate⁻ + H₂O	−39	7
	2H₂ (4H) + 2 acetate⁻ + H⁺ → butyrate⁻ + 2H₂O	−48	8
	10H₂ (20H) + 4HCO₃⁻ + 3H⁺ → butyrate⁻ + 10H₂O	−257	9
	Leucine + 3H₂O → isovalerate⁻ + HCO₃⁻ + H⁺ + NH₄⁺ + 2H₂	+4.2	10
	5 leucine + 5H₂O → 5 isovalerate⁻ + butyrate⁻ + 5NH₄⁺ + HCO₃⁻ + 2H⁺	−236	11
	Valine + 3H₂O → isobutyrate⁻ + HCO₃⁻ + H⁺ + NH₄⁺ + 2H₂	+9.7	12

Calculated from references 21 and 22.