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. 2021 Jun 24;16(1):919–930. doi: 10.1515/med-2021-0253

Figure 5.

Figure 5

The inhibition of miR-493-3p or miR-410-3p could restore the reverse effects of TUG1 knockdown on OGD/R-mediated decrease in viability and increase in apoptosis in N2a cells in vitro. (a and b) The effect of si-TUG1#2 and anti-miR-493-3p or anti-miR-410-3p on the expression of miR-493-3p or miR-410-3p was detected by RT-qPCR. (c) The effect of si-TUG1#2 and anti-miR-493-3p or anti-miR-410-3p on cell viability was evaluated by CCK-8 assay in N2a cells treated by OGD/R. (d) Flow cytometry assay was applied to detect the apoptosis rate in N2a cells, which transfected with si-TUG1#2 + anti-miR-493-3p or si-TUG1#2 + anti-miR-410-3p or negative controls, with or without being treated by OGD/R. (e and f) The proteins levels of apoptosis-related markers (cleaved-cas-3 and cleaved-cas-9) were performed by western blot assay in N2a cells, which transfected with si-TUG1#2 + anti-miR-493-3p or si-TUG1#2 + anti-miR-410-3p or negative controls, with or without being treated by OGD/R. *P < 0.05.