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. 2021 Jun 12;8(6):110. doi: 10.3390/vetsci8060110

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In ovo screening of feed compounds for their effect on embryonic growth, hatching, and peripheral lymphocyte numbers. (a) Effect of compound injections in embryos on the ratio of relative yolk and liver weight during late embryonic development, n = 6 per group per ED. (b) Effect of compound injections on hatchability (%) at ED21, n = 6 per group. (c) Effect of compound injections on the numbers of lymphocytes in whole blood in chicks immediately post-hatch. Non-injected eggs were included as the negative control and 0.4% saline solution as the solvent control, n = 4 per group. Mean + SEM is shown of compounds of polysaccharides (P1, P2, P8), simple sugars (SS2), modified sugars (MS3), and lipids (L1) in different concentrations (0.02, 0.2, 2%, or 0.0015, 0.0030, 0.0060 mg) and statistical significance is indicated as *** p < 0.001.