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. 2021 Jun 12;10(6):1484. doi: 10.3390/cells10061484

Figure 3.

Figure 3

Effect of XN on the endogenous and exogenous production of TIMP-1 and TIMP-2 in PMA-induced A549 cells. (a) After the 4-h pre-treatment with XN followed by 24-h co-incubation with PMA, the levels of TIMP-1 and TIMP-2 were determined by immunoblotting. (b) The intensity of protein bands from Western blot was quantified by densitometry analysis, where the untreated PMA-induced cells represented 100%. The results are presented as the mean ± SD of three independent experiments, ** p < 0.01, in comparison to the PMA-only treatment, ## p < 0.01 in comparison to the non-stimulated cells; one-way ANOVA test. (c) Following the XN treatment, the supernatants from the A549 cell cultures were collected and the levels of TIMP-1 and TIMP-2 were assayed with ELISA. The results are the mean ± SD of three independent experiments, *** p < 0.001 in comparison to the PMA-only treatment, ### p < 0.001 in comparison to the non-stimulated cells; one-way ANOVA test.