Inoculation of Viburnum opulus roots with Py. kashmirense. (A,B) Healthy seedlings of Viburnum opulus; (C) Healthy root system of Viburnum opulus cleaned with sterile distilled water; (D,E) Viburnum opulus roots after inoculation with Pythium kashmirense in vitro, showing discoloration and necrosis on around the 40 to 50% of the root segments; (F) Data analysis of Viburnum opulus Root Disease Severity in the in vitro assay; (G) Data analysis of Viburnum opulus Root Disease Severity in the in vivo assay; (D,E) Viburnum opulus roots after inoculation with Pythium kashmirense in vitro, showing discoloration and necrosis on around the 40 to 50% of the root segments; (F) Data analysis of Viburnum opulus Root Disease Severity in the in vitro assay; (G) Data analysis of Viburnum opulus Root Disease Severity in the in vivo assay. ** means p ≤ 0.01, **** means p ≤ 0.0001.