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. 2021 Jun 12;13(6):2030. doi: 10.3390/nu13062030

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PICRUSt2 functional prediction. (A) PCoA plot displaying Bray Curtis beta diversity matrix calculations (Control = orange; HA 35 KDa = blue). Percent confidence values for each distance matrix are displayed on the axes in two dimensions. PERMANOVA results: F = 1.80; p = 0.1. (B) Predicted metabolic pathways statistically attributed to the experimental variable. PICRUSt was used to predict the functional potential of microbiomes using 16S rRNA gene sequence data. Red bars represent controls; green bars represent HA 35 KDa-treated pups. HA: hyaluronic acid; PCoA: principal components analysis; LDA = linear discriminant analysis; PICRUSt = Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.