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. 2021 Jun 15;1(8):1147–1157. doi: 10.1021/jacsau.1c00139

Figure 2.

Figure 2

RNA sequence influences the binding stoichiometry to N protein. (A) Mass spectrum of N1–209 after incubation with 4 × −GAUGG RNA oligonucleotides in a molar ratio of 1:4 RNA:protein. Two additional charge state distributions are observed that correspond to one and two RNA oligonucleotides bound to N1–209. The mass spectrum at m/z > 2700 was magnified 3× and offset for clarity (red trace). (B) Mass spectrum of N1–209 after incubation with 4 × −GAGAA RNA oligonucleotides in a molar ratio of 1:4. One additional charge state distribution is observed that corresponds to one RNA oligonucleotide bound to N1–209. The mass spectrum at m/z > 2700 was magnified 3× and offset for clarity (red trace). (C) Mass spectrum of NFL after incubation with 4 × −GAUGG RNA oligonucleotides in a molar ratio of 1:4. Monomers and dimers of NFL (red circles) and N156–419 (blue circles) are observed. An additional peak series between 4300 and 5600 m/z corresponds to two 4 × −GAUGG RNA oligonucleotides bound to NFL dimer. The scheme in the inset of (C) depicts NFL dimer bound to RNA as the functional unit of the ribonucleoprotein assembly.