Table 2. Deconvoluted and Sequence Masses for N Proteoform Complexes.
| protein/complex | deconvoluted massa ± s.d. (Da) | expected mass (Da) |
|---|---|---|
| 4 × −GAUGG RNA | -- | 6622.00 |
| 4 × −GAGAA RNA | -- | 6650.20 |
| N1–209 + one 4 × −GAUGG RNA | 29 233 ± 0.4 | 29 234.71 |
| N1–209 + two 4 × −GAUGG RNA | 35 917 ± 10 | 35 856.71 |
| N1–209 + one 4 × −GAGAA RNA | 29 262 ± 1 | 29 262.91 |
| NFL dimer + two 4 × −GAUGG RNA | 105 131 ± 60 | 104 783.66 |
| cyclophilin A (CypA) | 20 084 ± 0.4 | 20 175.82 |
| 20 220 ± 0.8 | ||
| CypA dimers | 40 373 ± 62 | 40 351.64 |
| N1–209 + CypA | 42 867 ± 15 | 42 696.71 |
| N1–209 dimers | 45 356 ± 15 | 45 225.42 |
| cyclosporin A | 1202.85 ± 0 | 1202.63 |
| CypA + CsA | 21 287 ± 0.5 | 21 286.63 |
| mAb (monomer) | 137 990 ± 125 | -- |
| 145 193 ± 109 | ||
| NFL + mAb | 183 931 ± 102 | 183 759 |
| 190 962 | ||
| 2NFL + mAb | 229 984 ± 36 | 229 528 |
| 236 731 |
a
Determined using at least three adjacent charge states.