Table 3.
Frequency of meiotic abnormalities in the glyphosate- or glufosinate-tolerant fourth-generation progenies of the first backcross generation.
Stage | BC1mF4R | BC1pF4R | BC1mF4L | BC1pF4L | ||||
---|---|---|---|---|---|---|---|---|
NCO | F (%) | NCO | F (%) | NCO | F (%) | NCO | F (%) | |
MetaphaseI | 842 | 28.76 | 692 | 44.57 | 1,291 | 62.16 | 1,465 | 44.63 |
AnaphaseI | 573 | 11.90 | 784 | 27.07 | 661 | 60.36 | 736 | 64.52 |
TelophaseI | 795 | 1.59 | 887 | 2.35 | 807 | 11.60 | 647 | 10.51 |
MetaphaseII | 942 | 36.80 | 1,068 | 47.73 | 763 | 50.95 | 768 | 46.11 |
AnaphaseII | 759 | 9.24 | 836 | 15.90 | 925 | 72.63 | 692 | 42.35 |
TelophaseII | 1,054 | 3.77 | 1,100 | 2.93 | 935 | 21.13 | 980 | 14.61 |
NOC means the number of cells observed. F means the frequency of meiotic abnormalities.
BC1mF4R and BC1pF4R indicate the glyphosate-tolerant fourth-generation progenies of the first backcross generation (BC1) obtained from wild Brassica juncea × F1R or F1R × wild B. juncea, respectively. BC1mF4L and BC1pF4L indicate the glufosinate-tolerant fourth-generation progenies of the first backcross generation (BC1) obtained from wild Brassica juncea × F1L or F1L × wild B. juncea, respectively.
F1R and F1L indicate glyphosate- or glufosinate-tolerant F1 hybrids obtained from wild B. juncea × glyphosate- or glufosinate-tolerant transgenic oilseed rape. Progenitors in front of the × are always maternal plants, and progenitors after the × are always paternal plants.