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The bacterium Cutibacterium granulosum forms acetyl-CoA only when provided with all four substrates of the SCACT/SCS pathway. In different treatments, substrates were provided (+) or withheld (−). Results are means ± standard errors for 3 biological replicates. Means different from 0 (P < 0.05) are marked with an asterisk. Abbreviations: CoA, coenzyme A; SCACT, succinyl-CoA:acetate CoA-transferase; SCS, succinyl-CoA synthetase (ADP forming).
