FIG 6.

Purified SCACT and SCS form a functional pathway. (A) Acetyl-CoA formation by SCACT and SCS from succinate, acetate, ATP, and CoA. (B) ATP formation by SCACT and SCS from succinate, acetyl-CoA, and ADP. In different treatments, enzymes or substrates were provided (+) or withheld (−). Each milliliter of assay mix contained 4.5 μg for SCACT and 4.5 μg for SCS. Results are mean ± standard error of 3 biological replicates (batches of SCACT and SCS prepared from independent cultures). Means different from 0 (P < 0.05) are marked with an asterisk. Abbreviations: CoA, coenzyme A; SCACT, succinyl-CoA:acetate CoA-transferase; SCS, succinyl-CoA synthetase (ADP forming).