TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Description	Source or reference
Strains
Streptomyces strains
S. hygrospinosus subsp. beijingensis	Wild-type, anisomycin-producing strain	ACCC40033 (Agricultural Culture Collection of China)
ΔaniI	aniI deletion mutant	15
ΔaniI-C	aniI-complementary mutant	This study
ΔaniI::pJTU824	Control strain of ΔaniI-C	This study
WT::pOST-aniI	aniI overexpression strain under the control of the kasOp* promoter	This study
WT::pIB139-aniI	aniI overexpression strain under the control of the ermE* promoter	This study
WT::pOST	Control strain of WT::pOST-aniI	This study
WT::pIB139	Control strain of WT::pIB139-aniI	This study
E. coli strains
DH10B	General molecule cloning strain	Gibco BRL
BL21(DE3)	Host for protein expression	Stratagene
ET12567/pUZ8002	Intergeneric conjugation strain	44
Plasmids
pJTU824	Integrating vector containing ermE* promoter	45
pJTU824-aniI	Derivate plasmid of pJTU824 with intact gene aniI under the control of the ermE* promoter and used for complementation	This study
pET28a	Protein expression vector	Novagen
pET28a-aniI	Derivate pET28a plasmid containing the intact gene aniI	This study
pSET152	Integrative plasmid used for overexpression	46
pOST	Derived from pSET152 containing kasOp* promoter	This study
pOST-aniI	Derivate plasmid of pOST with intact gene aniI under the control of the kasOp* promoter and used for overexpression	This study
pIB139	Derived from pSET152 containing ermE* promoter	47
pIB139-aniI	Derivate plasmid of pSET152 with intact gene aniI under the control of the ermE* promoter and used for overexpression	This study