TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) | Use(s) |
|---|---|---|
| 824-I-fw | GTTGGTAGGATCCACATATGATGAAACCGACGCCCCCCGA (NdeI site) | Complementation of ΔaniI |
| 824-I-rv | TATGACATGATTACGAATTCTCACAGCTCCCGGACGATGC (EcoRI site) | |
| thioF | ATGCGGGGATCGACCGCGCG | Validation of ΔaniI-C |
| thioR | TCATCAGCTGCATACCGCTG | |
| I-A | AGCAAATGGGTCGCGGATCCATGAAACCGACGCCCCCCGA (BamHI site) | Overexpression of His-tagged AniI protein in E. coli |
| I-S | TGTCGACGGAGCTCGAATTCTCACAGCTCCCGGACGATGC (EcoRI site) | |
| pSET152-pkasOp-F | GGGCTGCAGGTCGACTCTAGATGTTCACATTCGAACGGTCTCTG (XbaI site) | Overexpression of aniI in WT strain |
| pSET152-pkasOp-R | CGCGGCCGCGGATCCTCTAGACTCCCCCAGTCCTGCACG (XbaI site) | |
| pSET152-AniI-F | TGGGGGAGTCTAGAGGATCCATGAAACCGACGCCCCCCGA (BamHI site) | Overexpression of aniI in WT strain |
| pSET152-AniI-R | TCGATATCGCGCGCGGCCGCCATATGTCACAGCTCCCGGACGATGC (NotI site) | |
| pIB139-AniI-F | GTTGGTAGGATCCACATATGATGAAACCGACGCCCCCCGA (NdeI site) | Overexpression of aniI in WT strain |
| pIB139-AniI-R | GCGGCCGCGGATCCTCTAGATCACAGCTCCCGGACGATGC (XbaI site) | |
| 16S-RT-F | CCGCAAGGCTAAAACTCAAA | Detection of the transcriptional level of 16S rRNA |
| 16S-RT-R | AACCCAACATCTCACGACAC | |
| AniI-RT-F | CCCCGCTTCCTGTGCGAGTT | Detection of the transcriptional level of aniI |
| AniI-RT-R | GGTGGTTGGCGGTGAAGAGC |