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. 2021 Jun 13;22(12):6340. doi: 10.3390/ijms22126340

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Monocyte adhesion and activation on polymer surfaces. Human peripheral blood mononuclear cells (PBMCs) were isolated from an in-line filter system for leukocyte filtration. Subsequently, PBMCs were exposed to the indicated polymer surfaces for 3 h. After incubation, adherent PBMCs were stained for DAPI (nucleus) and CD14, which is expressed by monocytes. Representative images of 5 independent experiments are shown. Images were quantified using ImageJ software. Bars show mean ± SD of adherent monocytes compared to the PLLA surface with * p < 0.05, ** p < 0.01 and *** p < 0.0001.